Selenium and Selenoproteins
Aging & Lifespan Control
Welcome to Our Lab
- Redox Biology
- Selenium and Selenoproteins
- Aging & Lifespan Control
- Cancer Prevention
- Comparative Genomics
Feature in the NIH Director's BlogDr. Collins features the Gladyshev lab's research in his blog post.
Last Updated: Thursday, 2 April 2015
- Monitoring methionine sulfoxide with stereospecific mechanism-based fluorescent sensors:
- Methionine can be reversibly oxidized to methionine sulfoxide (MetO) under physiological and pathophysiological conditions, but its use as a redox marker suffers from the lack of tools to detect and quantify MetO within cells. In this work, we created a pair of complementary stereospecific genetically encoded mechanism-based ratiometric fluorescent sensors of MetO by inserting a circularly permuted yellow fluorescent protein between yeast methionine sulfoxide reductases and thioredoxins. The two sensors, respectively named MetSOx and MetROx for their ability to detect S and R forms of MetO, were used for targeted analysis of protein oxidation, regulation and repair as well as for monitoring MetO in bacterial and mammalian cells, analyzing compartment-specific changes in MetO and examining responses to physiological stimuli.
- Monitoring methionine sulfoxide with stereospecific mechanism-based fluorescent sensors. Nat Chem Biol., In press.
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