• Redox Biology
  • Selenium and Selenoproteins
  • Aging & Lifespan Control
  • Cancer Prevention
  • Comparative Genomics

Feature in the NIH Director's Blog

Dr. Collins features the Gladyshev lab's research in his blog post.

Last Updated: Thursday, 9 July 2015

Featured Publication

  • Monitoring methionine sulfoxide with stereospecific mechanism-based fluorescent sensors:
  • Methionine can be reversibly oxidized to methionine sulfoxide (MetO) under physiological and pathophysiological conditions, but its use as a redox marker suffers from the lack of tools to detect and quantify MetO within cells. In this work, we created a pair of complementary stereospecific genetically encoded mechanism-based ratiometric fluorescent sensors of MetO by inserting a circularly permuted yellow fluorescent protein between yeast methionine sulfoxide reductases and thioredoxins. The two sensors, respectively named MetSOx and MetROx for their ability to detect S and R forms of MetO, were used for targeted analysis of protein oxidation, regulation and repair as well as for monitoring MetO in bacterial and mammalian cells, analyzing compartment-specific changes in MetO and examining responses to physiological stimuli.
  • Tarrago L, Peterfi Z, Lee BC, Michel T, Gladyshev VN. (2015) Monitoring methionine sulfoxide with stereospecific mechanism-based fluorescent sensors. Nat Chem Biol. 11, 332-338.
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